This tool predicts the most probable miRNA duplex on a given miRNA hairpin.You can upload a txt file with your sequences in fasta format, (include fasta file header). The output is presented as a three-column tab-delimited table. The first column lists the sequence IDs as specified by the user, while the second and third columns display the most probable matures from the 5’ and the 3’ strands of the hairpin, respectively. Entries for which the RNAfold output is not a hairpin or consists of multiple stems are filtered out, and “Multibranch - No prediction” is returned as an output for the respective lines. If you wish to receive your results via email, you can provide your email address (optional). Input example: >MI0000060 UGGGAUGAGGUAGUAGGUUGUAUAGUUUUAGGGUCACACCCACCACUGGGAGAUAACUAUACAAUCUACUGUCUUUCCUA >MI0000061 AGGUUGAGGUAGUAGGUUGUAUAGUUUAGAAUUACAUCAAGGGAGAUAACUGUACAGCCUCCUAGCUUUCCU >MI0000065 CCUAGGAAGAGGUAGUAGGUUGCAUAGUUUUAGGGCAGGGAUUUUGCCCACAAGGAGGUAACUAUACGACCUGCUGCCUUUCUUAGG >MI0003603 UCUUAUCAAUGAGGUAGACCAUGGGUUCUCAUUGUAAUAGUGUAGAAUGUUGGUUAACUGUGGACUCCCUGGCUCUGUCUCAAAUCUACUGAUUC >MI0000070 GUCAGCAGUGCCUUAGCAGCACGUAAAUAUUGGCGUUAAGAUUCUAAAAUUAUCUCCAGUAUUAACUGUGCUGCUGAAGUAAGGUUGAC >MI0003681 GUGUAGUAGAGCUAGGAGGAGAGGGUCCUGGAGAAGCGUGGACCGGUCCGGGUGGGUUCCGGCAGGUUCUCACCCUCUCUAGGCCCCAUUCUCCUCUG >MI0000071 GUCAGAAUAAUGUCAAAGUGCUUACAGUGCAGGUAGUGAUAUGUGCAUCUACUGCAGUGAAGGCACUUGUAGCAUUAUGGUGAC Output example: equenceID 5strandMature 3strandMature MI0000060 UGAGGUAGUAGGUUGUAUAGUU CUAUACAAUCUACUGUCUUUC MI0000061 UGAGGUAGUAGGUUGUAUAGUU CUGUACAGCCUCCUAGCUUUCC MI0000065 AGAGGUAGUAGGUUGCAUAGUU CUAUACGACCUGCUGCCUUUC MI0003603 Multibranch - No prediction MI0000070 UAGCAGCACGUAAAUAUUGGCG CCAGUAUUAACUGUGCUGCUGA MI0003681 UGGAGAAGCGUGGACCGGUCC CAGGUUCUCACCCUCUCUAGG MI0000071 CAAAGUGCUUACAGUGCAGGUAG ACUGCAGUGAAGGCACUUGUAG